The chromatographic system first separates the different components, concentrating the amount of each single component reaching the mass spectrometer. The first quadrupole of the triple quadrupole configuration ionizes the molecules of the analyte. Selected molecular ions are then fragmented in the second quadrupole and selectively isolated by the third and final quadrupole for measurement by a detector. This series of separation, ionization and selective fragmentation provides highly sensitive detection.
McCrone Associates has expanded their suite of mass spectrometry instrumentation. Additionally, accurate quantitation is possible for some sample matrices. Materials Analysis. EAG has unparalleled experience with the operation and application of this powerful technique to quantify a wide variety of compounds in a wide variety of matrices.
EAG employs the latest instrumentation with industry leading standards for sensitivity and accuracy. To enable certain features and improve your experience with us, this site stores cookies on your computer.
Please click Continue to provide your authorization and permanently remove this message. Ask EAG. Liquid Chromatography - Tandem Mass Spectrometry LC-MS-MS Liquid Chromatography with tandem mass spectrometry LC-MS-MS is a powerful analytical technique that combines the separating power of liquid chromatography with the highly sensitive and selective mass analysis capability of triple quadrupole mass spectrometry. Ideal Uses Trace analysis in: Environmental samples i.
If two mass spec detectors are used, then molecules with a particular mass-to-charge ratio can be chosen to undergo further analysis by fragmenting the ion by collision-induced dissociation or other fragmentation processes. These fragmented ions can then be detected by the second mass spec unit. The mass spec detector transfers the mass-to-charge data to a computer which graphical presents the information as a mass spectrum. The mass spectrum of the sample can be used to determine the concentration of known or unknown compounds, find the mass of impurities and give insight into chemical structures.
Glycosylation and other post-translational modifications can be analyzed by LC-MS. LC-MS is a convenient method for identity testing using a reference standard and to determine purity of drug product or drug substance. Plastics from pharmaceutical packaging or exposed to the drug substance or drug product during the manufacturing process could leach into the product and potentially cause toxic side effects.
0コメント